ThemeDevelopmental Biotechnology and Protozoan Infection
|Keyword||Dog, Mouse, Protozoa|
I focus on host-parasite interactions with modified physiological condition of host animals using the transgenic technology. A target for protozoan infection might be host animals as well as parasites. In addition, techniques for reproductive bio-technology, one of the basic techniques of transgenic technology, are developing in the Research Unit for Functional Genomics.
Alpha-tocopherol transfer protein (α-TTP) is known to be a determinant of the plasma concentration of α-tocopherol (vitamin E), a lipid soluble ant-oxidant, in the body. Thus, α-TTP gene disruption induces vitamin E deficiency, undetectable level of vitamin E in circulation, in animals. When α-TTP knockout mice were infected with murine malarial parasites such as Plasmodium berghei NK65 and P. yoelli, their survival after the infection significantly extended as compared with wild type animals. The resistance phenotype against the protozoan infection were also seen in α-TTP knockout mice inoculated with trypanosoma congolense, a causing agent of Sura in domestic animals. Biochemical and molecular biological analysis revealed that proliferation of the infected parasites were inhibited by DNA damage derived from oxidative stress in circulation. In addition, α-TTP knockout mice infected with P. berghei ANKA, known to be able to induce cerebral malaria in mice, did not show any typical clinical symptom regarding cerebral malaria including blood brain barrier breakdown. These results indicate that inhibition of α-TTP activity might be a new approach for prevention and treatment of the protozoan infection in both human and other mammalian species.
Our techniques in reproductive bio-technology are applied for the improved breeding of the guide dogs for the blind people. Many countries including Japan suffer from an acute shortage of guide dogs. We have developed a novel cryoprotectant for canine spermatozoa for extension of artificial insemination technique in guide dog in Japan. More recently, we have successfully produced seven puppies derived from frozen embryos after the embryo transfer for the first time in the world. furthermore, control of zoonotic infection between dogs and humans may be an important factor for distribution and extension of the guide dog for the blind.
List of current research topics
- Effect of vitamin E defficiency on protozoan infection.
- Analysis of pregnant malaria in mice.
- Analysis of reproductive ability in male infected with malaria.
- Cryopreservation of emmbryo, sperm and ovary in dog.
- Establishment of Babesia resistant cattle.
|Room address||National Research Center for Protozoan Diseases|
|Mail address||hisuzuki obihiro.ac.jp|
BelongsNational Research Center for Protozoan Diseases/Department of Drug Discovery and Development/Research Unit for Innovative Medicine Research Center for Global Agromedicine/Department of Veterinary MedicineVeterinary Medical Center/Diagnostic Resouce
|Affiliated academic society||Japanese Society for Ova Research, The Japanese Society of Parasitology The Jamanese Society of Veterinary Science, Japan Society for Reproductive Medicine, The Molecular Biology Society of Japan, Japan Society of Tropical Medicine, Japanese Society of Animal Reproduction, The Japanese Society for Genome Editing, Japan Society of Service Dog Research, Japanese Association for Laboratory Animal Science, The Japanese Association for Laboratory Animal Facillities of National University Corporations, Society for the Stude of Reproduction|
|Academic background||1979-1983 School of Veterinary Medicine and Animal
Science, Kitasato University
Awarded the degree of BSc. in Agriculture
1983-1985 Department of Animal Breeding and
Reproduction, Institute of Veterinary
Medicine and Animal Science, Kitasato
Awarded the degree of MSc. in Agriculture
for a thesis entitled "Studies on the
development of mouse embryos derived from
fertilization in vitro". Work supervised by
Professor Yutaka Toyoda
1995 Department of Reproductive and Developmental
Biology, Institute of Medical Science,
University of Tokyo
Awarded the degree of Ph.D in veterinary
medicine for a thesis entitled "Studies on
the development of zona-free mouse embryos".
Work supervised by Professor Yutaka Toyoda
1985-1997 Researcher at Chugai Pharmaceutical Co.,
1997-2001 Senior Researcher at Chugai Pharmaceutical
2001-present Professor at Research Unit for Functional
National Research Center for Protozoan
Diseases, Obihiro University of
Agriculture and Veterinary Medicine
2002-2006 Guest Professor at Department of Developmental
and Medical Technology, Graduate School of
Medicine, The University of Tokyo
1996-2001 Guest researcher at National Institute of
Radiological Sciences, Japan
2003-2007 Advisor to the president at Obihiro University
2010-2014 Director General at National Research Center for Protozoan Diseases, Obihiro University of
Agriculture and Veterinary Medicine