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National Research Center for
Protozoan Diseases

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Shin-ichiro Kawazu: Research Activities
Publications (2008~Current)

Dang-Trinh MA, Angeles JMM, Moendeg KJ, Macalanda AMC, Higuchi L, Oto C, Kirinoki M, Chigusa Y, *Kawazu SI. Utilization of real time PCR for the assessment of egg burden in the organs of Schistosoma japonicum experimentally infected mice. Exp Parasitol. 189:61-65. doi: 10.1016/j.exppara.2018.04.014 (2018).

Asada M, Hakimi H, *Kawazu SI. The application of the HyPer fluorescent sensor in the real-time detection of H2O2 in Babesia bovis merozoites in vitro. Vet Parasitol. doi: 10.1016/j.vetpar.2018.03.016 (2018).

Macalanda AMC, Angeles JMM, Moendeg KJ, Dang AT, Higuchi L, Inoue N, Xuan X, Kirinoki M, Chigusa Y, Leonardo LR, Villacorte EA, Rivera PT, Goto Y, *Kawazu SI. Evaluation of Schistosoma japonicum thioredoxin peroxidase-1 as a potential circulating antigen target for the diagnosis of Asian schistosomiasis. J Vet Med Sci. doi: 10.1292/jvms.17-0579 (2017).

*Yamagishi J, Asada M, Hakimi H, Tanaka TQ, Sugimoto C, Kawazu S. Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens. BMC Genomics. doi: 10.1186/s12864-017-4230-4 (2017).

Moendeg KJ, Angeles JMM, Nakao R, Leonardo LR, Fontanilla IKC, Goto Y, Kirinoki M, Villacorte EA, Rivera PT, Inoue N, Chigusa Y, *Kawazu SI. Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers. PLoS Negl Trop Dis. 11(7):e0005749 (2017).

Hakimi H, Yamagishi J, Kegawa Y, Kaneko O, *Kawazu S, *Asada M. Establishment of transient and stable transfection systems for Babesia ovata. Parasit Vectors. doi: 10.1186/s13071-016-1439-z (2016.)

Angeles JM, Leonardo LR, Goto Y, Kirinoki M, Villacorte EA, Hakimi H, Moendeg KJ, Lee S, Rivera PT, Inoue N, Chigusa Y, *Kawazu S. Water buffalo as sentinel animals for schistosomiasis surveillance. Bull World Health Organ. doi: 10.2471/BLT.14.143065 (2015).

Mirano-Bascos, D., Tongol-Rivera, P., Villacorte, EA., Escueta, AD., Kawazu, S., and *Kano S. Characterization of the population demographics and the MSP-1 block 2 allele gene frequencies of P. falciparum infected individuals in Davao, Philippines. Trop. Med. Health 41(1):1-6 (2013)

Turturice, BA., Lamm, MA., Tasch, JJ., Zalewski, A., Kooistra, RL., Schroeter, EH., Sharma, S., Kawazu, SI., and *Kanzok SM. Expression of cytosolic peroxiredoxins in Plasmodium berghei ookinetes is regulated by environmental factors in the mosquito bloodmeal. PLoS Pathog. 9(1):e1003136 (2013)

Angeles, JM., Kirinoki, M., Goto, Y., Asada, M., Hakimi, H., Leonardo, L., Tongol-Rivera, PT., Villacorte, EA., Inoue, N., Chigusa, Y., and *Kawazu, S. Localization and expression profiling of a 31kDa antigenic repetitive protein Sjp_0110390 in Schistosoma japonicum life stages. Mol. Biochem. Parasitol. 187: 98-102 (2013)

Usui, M., Masuda-Suganuma, H., Fukumoto, S., Angeles, JM., Inoue, N., and *Kawazu, S. Expression profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium berghei. Parasitol. Int. [Epub ahead of print] (2012)

Kimura, R., Komaki-Yasuda, K., Kawazu, S., and *Kano, S. 2-Cys peroxiredoxin of Plasmodium falciparum is involved in resistance to heat stress of the parasite. Parasitol. Int. 62: 137-143 (2012)

Suganuma, K., Yamasaki, S., Asada, M., Kawazu, S., and *Inoue, N. S. The epimastigote stage-specific gene expression of CESP is tightly regulated by its 3’UTR. Mol. Biochem. Parasitol. 186: 77-80 (2012)

Hakimi, H., Asada, M., Angeles, JM., Inoue, N., and *Kawazu, S. Plasmodium vivax and Plasmodium knowlesi: Cloning, expression and functional analysis of 1-Cys peroxiredoxin. Exp. Parasitol. 133: 101-105 (2012)

Masuda-Suganuma, H., Usui, M., Fukumoto, S., Inoue, N., and *Kawazu, S. Mitochondrial peroxidase TPx-2 is not essential in the blood and insect stages of Plasmodium berghei. Parasit. Vectors 5: 252 (2012)

Ybañez, AP., Sivakumar, T., Ybañez, RH., Ratilla, JC., Perez, ZO., Gabotero, SR., Hakimi, H., Kawazu, S., Matsumoto, K., Yokoyama, N., and *Inokuma, H. First molecular characterization of Anaplasma marginale in cattle and Rhipicephalus (Boophilus) microplus ticks in Cebu, Philippines. J. Vet. Med. Sci. Aug 10. [Epub ahead of print] (2012)

Angeles, JM., Goto, Y., Kirinoki, M., Asada, M., Leonardo, L., Rivera, PT., Villacorte, E., Inoue, N., Chigusa, Y.,  and *Kawazu, S. Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes. PLoS Neglected Tropical Diseases 6 (8): e1800 (2012)

Enomoto, M., Kawazu, S. Kawai, S., Fruyama, Y., Ikegami T., Watanabe, J., and *Mikoshiba, K. Blockage of spontaneous Ca2+ oscillation causes cell death in intraerythrocitic Plasmodium falciparum. PLoS ONE (2012) in press.

Asada, M., Goto, Y., Yahata, K., Yokoyama, N., Kawai, S., Inoue, N., Kaneko, O., and *Kawazu, S. Gliding Motility of Babesia bovis merozoites visualized by time-lapse video microscopy. PLoS ONE 7: e35227 (2012)

*Kimura, A., Nishikawa, H., Nomura, N., Mitsuyama, J., Fukumoto, S., Inoue, I., and Kawazu, S. In vitro and in vivo antimalarial activity of T-2307, a novel arylamidine. Antimicrob. Agents Chemother. 56: 2191-2193 (2012)

Hakimi, H., Asada, M., Angeles, JM., Inoue, N., and *Kawazu, S. Coning and characterization of Plasmodium vivax thioredoxin peroxidase-1. Parasitol. Res. 2012 (in press)

Asada, M., Tanaka, M., Goto, Y., Yokoyama, N., Inoue, N., and *Kawazu, S. Stable expression of green fluorescent protein and targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis with the WR99210/dhfr selection system. Mol. Biochem. Parasitol. 181: 162-170 (2012)

*Goto, Y., Duthie MS., Nguyen, TT., Asada, M., Kawazu, S., Carter, D., and Inoue, N. Serological characterizations of tandem repeat proteins for detection of African trypanosome infection in cattle. Parasitol. Int. 60: 538-540 (2011)

Angeles, JM., Goto, Y., Kirinoki, M., Leonardo, L., Rivera, PT., Villacorte, E., Inoue, N., Chigusa, Y.,  and *Kawazu, S. Human antibody response to thioredoxin peroxidase-1 and tandem repeat proteins as immunodiagnostic antigen candidates for Schistosoma japonicum infection. Am. J. Trop. Med. Hyg. 85: 674-679 (2011)

Nguyen, TT., Goto, Y., Lun, ZR., Kawazu, S., and *Inoue, N. Tandem Repeat Proteins as potential diagnostic antigen for Trypanosoma evansi infection. Parasitol. Res. 110: 733-739 (2012)

Laohasinnarong, D., Thekisoe, OM., Malele, I., Namangala, B., Ishii, A., Goto, Y., Kawazu, S., Sugimoto, C., and *Inoue, N. Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP). Parasitol. Res. 109: 1735-1739 (2011)

Usui, M., Fukumoto, S., Inoue, N., and *Kawazu, S. Improvement of the observational method for Plasmodium berghei oocysts in the midgut of mosquitoes. Parasit. Vectors 4: 118 (2011)

Khukhuu, A., Lan, DT., Long, PT., Ueno, A., Li, Y., Luo, Y., Macedo, AC., Matsumoto, K., Inokuma, H., Kawazu, S., Igarashi, I., Xuan, X., and *Yokoyama, N. Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue Province, Vietnam. J. Vet. Med. Sci. 73: 701-705 (2011)

*Goto, Y., Duthie, MS., Kawazu, S., Inoue, N., and Carter, D. Biased cellular locations of tandem repeat antigens in African trypanosomes. Biochem. Biophys. Res. Commun. 405: 434-438 (2011)

Thekisoe, OM., Rodriguez, CV., Rivas, F., Coronel-Servian, AM., Fukumoto, S., Sugimoto, C., Kawazu, S., and *Inoue, N. Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP). Am. J. Trop. Med. Hyg. 82: 855-860 (2010)

*Kawazu, S., Takemae, H., Komaki-Yasuda, K., and Kano, S. Target proteins of the cytosolic thioredoxin in Plasmodium falciparum. Parasitol. Int. 59: 298-302 (2010)

*Goto, Y., Carter, D., Guderian, J., Inoue, N., Kawazu, S., and Reed, SG. Up-regulated expression of a B-cell antigen family tandem repeat proteins by Leishmania amastigotes. Infect. Immun. 78: 2138-2145 (2010)

Hikosaka, K., Watanabe, YI., Tsuji, N., Kita, K., Kishine, H., Arisue, N., Palacpac, NM., Kawazu, S., Sawai, H., Horii, T., Igarashi, I., and *Tanabe, K. Divergence of the mitochondrial genome structure in the apicomplexan parasites, Babesia and Theileria. Mol. Biol. Evol. 27: 1107-1116 (2010)

Thekisoe, OM., Rambritch, NE., Nakao, R., Bazie, RS., Mbati, P., Namangala, B., Malele, I., Skilton, RA., Jongejan, F., Sugimoto, C., Kawazu, S., and *Inoue, N. Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes. Int. J. Parasitol. 40: 55-61 (2010)
Tanaka, M., Sakurai, T., Yokoyama, N., Inoue, N., and *Kawazu, S. Cloning and characterization of peroxiredoxin in Babesia bovis. Parasitol. Res. 105: 1473-1477 (2009)

Terkawi, MA., Amornthep, A., Ooka, H., Aboge, G., Jia, H., Goo, YK., Nelson, B., Yamagishi, J., Nishikawa, Y., Igarashi, I., Kawazu, S., Fujisaki, K., and *Xuan, X. Molecular characterizations of three distinct Babesia gibsoni rhoptry-associated protein-1s (RAP-1s). Parasitology 136: 1147-1160 (2009)

Hatabu, T., Iwagami,M., Kawazu, S., Taguchi, N., Escueta, AD., Villacorte, EA., Rivera, PT., and *Kano, S. Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: A Philippine study. Parasitol. Int. 58: 166-170 (2009)

Terkawi, MA., Aboge, G., Jia, H., Goo, YK., Ooka, H., Yamagishi, J., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S., Fujisaki, K., and *Xuan, X. Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein-70. Parasite Immunol. 31: 328-40 (2009)

Iwagami, M., Rivera, PT., Villacorte, EA., Escueta, AD., Hatabu, T., Kawazu, S., Hayakawa, T., Tanabe, K., and *Kano, S. Genetic diversity and population structure of Plasmodium falciparum in the Philippines. Malar. J. 8: 96 (2009)

Thekisoe, OM., Bazie, RS., Coronel-Servian, AM., Sugimoto, C., Kawazu, S., and *Inoue, N. Stability of Loop-Mediated Isothermal Amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates. J. Vet. Med. Sci. 71: 471-475 (2009)

Sakurai, T., Tanaka, M., Kawazu, S., and *Inoue, N. Establishment of an in vitro transgene expression system in epimastigotes of Trypanosoma congolense. Parasitol. Int. 58:110-113 (2009)

Okado, K., Shinzawa, N., Aonuma, H., Nelson, B., Fukumoto, S., Fujisaki, K., Kawazu, S., and *Kanuka, H. Rapid recruitment of innate immunity regulates variation of intracellular pathogen resistance in Drosophila. Biochem. Biophys. Res. Commun. 379: 6-10 (2009)

*Saito-Nakano, Y., Tanabe, K., Kamei, K., Iwagami, M., Komaki-Yasuda, K., Kawazu, S., Kano, S., Ohmae, H., and Endo, T. Genetic evidence for Plasmodium falciparum resistance to chloroquine and pyrimethamine in Indochina and the Western Pacific between 1984 and 1998. Am. J. Trop. Med. Hyg. 79: 613-619 (2008)

Komaki-Yasuda, K., Okuwaki, M., Kano, S., Nagata, K., and *Kawazu, S. 5' sequence- and chromatin modification-dependent gene expression in Plasmodium falciparum erythrocytic stage. Mol. Biochem. Parasitol. 162: 40-51 (2008)

Terkawi, MA., Zhang, G., Jia, H., Aboge, G., Goo, YK., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S., Fujisaki, K., and *Xuan, X. C3 contributes to the cross-protective immunity induced by Babesia gibsoni phosphoriboprotein P0 against a lethal B. rodhaini infection. Parasite Immunol. 30: 365-370 (2008)

Yano, K., Otsuki, H., Arai, M., Komaki-Yasuda, K., Tsuboi, T., Torii, M., Kano, S., and *Kawazu, S. Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito. Mol. Biochem. Parasitol. 159:142-145 (2008)

Iwagami, M., Hatabu, T., Kawazu, S., Escueta, AS., Villacorte, EA., Tongol-Rivera, P., and *Kano S. Phylogenetic relationship of Plasmodium falciparum populations in the Philippines. Southeast Asian J. Trop. Med. Public Health 39: 200-204 (2008)

*Kawazu, S., Komaki-Yasuda, K., Oku, H., and Kano, S. Peroxiredoxins in malaria parasites: Parasitologic aspects. Parasitol. Int. 57: 1-7 (2008)

*:Corresponding author